Green synthesis of metallic nanoparticles by certain microorganisms and their applications

Paper ID : 1048-ISCH

Authors

Dalia Muhammad Abdelrahman * Master degree

Abstract

Nanotechnology is one of the most important technologies that enter into multiple fields. Silver-gold nanocomposite which gained a great interest nowadays. New Aspergillus fumigatus and Aspergillus flavus isolates have been isolated from a metal contaminated soil. Both isolates have reported good potential for Ag-Au nanocomposite production. Sphere-like polyhedron nanoparticles with 10-25 nm size range have been detected. Both samples recorded good surface charge value (-29.0 ± 2.3 & -27.3 ± 0.4) that can support a reasonable stability for the prepared nano-solution, Resulted of FTIR spectra of both nanocomposite samples showed many common bands between them. The Ag-Au nanocomposites synthesized by both fungal isolates have showed good biological activities. They exerted good inhibitory effect against all tested Gram-negative and Gram-positive bacteria. Furthermore, they showed significant antifungal behavior against different fungal plant pathogens, In a trial to evaluate the safe applicability of the biosynthesized Ag-Au nanocomposites and to estimate their anticancer potential, their cytotoxic effect against a normal and cancer cell line has been evaluated. The cytotoxicity revealed the lower toxicity of the biosynthesized Ag-Au nanocomposites on the mice bone marrow cells. While both samples exerted obvious toxic effect against MCF7 cell line with significant IC50 values. Ag-Au nanocomposite synthesized by A. flavus ND1 OR63649 showed higher toxicity to the MCF7 cells than that exerted by Ag-Au nanocomposite synthesized by A. fumigatus ND2 OR63649 where they have reported IC50 values of 25.94 and 35.31 µg/mL, respectively.

Keywords

Aspergillus fumigatus, Aspergillus flavus, Ag-Au nanocomposite, antimicrobial, cytotoxicity, green synthesis.

Status: Abstract Accepted (Poster Presentation)