| Synthesis and characterization of artificial seeds plantlets of in vitro male Populus alba |
| Paper ID : 1053-ISCH |
| Authors |
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Eman Tawfik Hussien * Associate Professor of Genetics and Genetic Engineering - Botany and Microbiology Department - Faculty of Science - Helwan University - Egypt |
| Abstract |
| Synthetic seeds are defined as any tissue with the potential to grow into a plant under in vitro or in vivo conditions that has been artificially encapsulated somatic embryos, shoot buds, cell aggregates, or other tissues. These tissues retain their potentiality even after being stored. Somatic embryos used in plant production and dispersed in the field were considered synthetic seeds. After using artificial seeds, shoot apical tips, axillary buds, and nodal segments have also been used as suitable substitutes for somatic embryos. The current study’s goal was to standardize artificial seed production methods using Populus alba encapsulated nodal explants and in vitro regeneration. This woody species is male tree and has no seed formation. Several concentrations of sodium alginate (2, 3, 4 and 5%) were mixed separately with the somatic endosperm of P. alba; and finally encapsulated with various concentrations of CaCl2 (50, 75, 100 and 200mM). The morphological and physiological parameters were measured and sometimes showed significant differences. The best conditions for these synthetic seeds’ formation based on the morphological and physiological parameters were the beads with 3 and 4% sodium alginate which are encapsulated by 100 and 200mM of CaCl2. The current study clearly established and demonstrated the best method for producing artificial seed in P. alba. |
| Keywords |
| Artificial seeds · Synthetic endosperm · Somatic embryogenesis · Hydrogel encapsulation · RAPD-PCR. |
| Status: Abstract Accepted (Oral Presentation) |