| Assessing the Anthelmintic Properties of Carica papaya Leaf Extracts: Insights from in vitro and in silico Investigations |
| Paper ID : 1059-ISCH |
| Authors |
|
Mona Fathi Khalil *1, Nawal Salem Alhaiqi2, Sherien Mostfa Afifi2, Jazem Abdullah Mahyoub3, Rewaida Abdel-Gaber4, Denis Delic5, Mohamed Abdel-Moneim Dkhil1 1Department of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt 2Department of Zoology, Faculty of Women for Arts, Sciences and Education - Ain Shams University. Egypt 3Department of Biological Sciences, Faculty of Science, King Abdul Aziz - University, Jeddah, Saudi Arabia 4Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia 5Translational Medicine & Clinical Pharmacology, Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach, Germany |
| Abstract |
| Helminthiasis remains a major global health concern. Exploring natural alternatives due to drug resistance and synthetic drug side effects is increasingly urgent. Therefore, This study investigates the anthelmintic potential of Carica papaya leaf extracts (CPLE) against Allolobophora caliginosa, along with elucidating the underlying structural alterations and molecular interactions. Carica papaya underwent methanolic extraction. Gas chromatography-mass spectrometry analysis revealed 11 active phytochemical compounds within CPLE. Anthelmintic activity was evaluated against A. caliginosa, with CPLE demonstrating efficacy comparable to albendazole. Light microscopy and scanning electron microscopy depicted structural modifications in worms exposed to CPLE, characterized by reduced size, uniform shrinkage, and increased cuticle thickness. Molecular docking studies with proteins Ascaris lumbricoides β-tubulin and Trichuris trichiura β-tubulin revealed potential binding interactions of CPLE compounds, notably Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) ethyl ester, and Albendazole oxide. These findings suggest the anthelmintic efficacy of CPLE and provide insights into its mode of action at the molecular level. |
| Keywords |
| Helminthiasis, Carica papaya, earth worms, Structural alterations, Molecular docking. |
| Status: Abstract Accepted (Poster Presentation) |