| Enhancing the wound healing process through topical treatment of exosomes derived stem cell |
| Paper ID : 1068-ISCH |
| Authors |
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Alaa Abdelrahman Hassanen *1, Ahmed Said Alazzouni2, Basma Nasr Hassan3, Doaa Ezz Soliman1 1Faculty of science Helwan University 2Department of zoology, faculty of science, Helwan university, Cairo, Egypt. 3Faculty of science, Helwan University |
| Abstract |
| The skin plays a crucial role as a protective barrier against external factors. Scar formation and delayed wound healing present significant challenges in skin injury treatment. Recently, exosomes have been considered the key substances involved in the healing of wounds. Aim of this work to evaluate the effect of exosomes in mice model of wound healing. 5 mm round -shaped full thickness of the excision wound was aseptically created on the dorsal region of each mouse using a sterilized punch biopsy (0.5 cm^2). Forty-five healthy adult male Swiss albino mice were divided into three groups: control group (five mice) and untreated wounded group (twenty mice), exosomes wounded group (topically treated with a thin layer of aqueous cream containing exosomes & twenty mice). Skin tissues formed at wound area were sampled for analysis on the 7, 14, 21, 45 days post-wounding (in untreated wounded group & exosomes wounded group) and five mice for each period were sacrificed by cervical dislocation. Histopathological examination including Hematoxylin and Eosin was performed and also Masson's Trichrome Staining for observation of collagen fibers deposition. Immunohistochemical examination including VEGF was performed. Our in vivo experiments demonstrated that the topical application of exosomes on excision wound accelerated the wound healing process, resulting in wound closure, collagen synthesis, vessel formation, and angiogenesis in the wound area. the inflammation is witnessed and rises in level of VEGF. Collectively, the obtained data depict that exosomes have a promising therapeutic potential for expediting wound healing and minimizing scar formation. |
| Keywords |
| Skin, mice, wound healing, exosome, histology, VEGF |
| Status: Abstract Accepted (Oral Presentation) |