| In vitro Cytotoxic Evaluation of Quercetin Loaded Lecithin/Chitosan Nanoparticles against Colorectal Cancer |
| Paper ID : 1081-ISCH |
| Authors |
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Ahmed Mohamed El-Sayed *1, Wafaa Ghoneim Shousha2, Mohamed Diaa El-Dein Abd El-Maksoud3, Shaimaa Shawki Ramadan4, Abdou Kamal Allayeh5 1Scholarship holder within the scientists for next generation (SNG) 8th batch provided by the Academy of Scientific Research and Technology (ASRT) 2Professor and Head of Chemistry Department, Faculty of Science, Helwan University 3Professor of Medical Biochemistry, Biochemistry Department, Biotechnology Research Institute, National Research Centre 4Associate Professor of Biochemistry, Chemistry Department, Faculty of Science, Helwan University 5Associate Professor of Virology, Water and Pollution Research Department, Environment and Climate Research Institute, National Research Centre |
| Abstract |
| Quercetin is a bioactive compound present abundantly in plants that possess potent anti-apoptosis, anti-cell proliferation, and anti-metastasis properties against colorectal cancer. However, owing to its low bioavailability and relative hydrophobicity, this flavonoid is difficult to utilize. Biopolymer nanoparticles offer small size, low-degradation, targeted, and sustained delivery systems, while polysaccharides offer reducing agents for stabilizing nanoparticles in an aqueous environment. Chitosan is a linear positively charged polysaccharide, with non-toxicity, biodegradability, biocompatibility, and anti-inflammatory properties. It is a preferred polyelectrolyte for coating negatively charged surfaces in nanoparticles. Lecithin which is a negatively charged phospholipid can improve the properties of the polymeric network formed by the opposite-charged biomolecules. The goal of this research was to encapsulate quercetin in lecithin/chitosan nanoparticles and characterize their physiochemical features in terms of mean size, polydispersity index (PDI), zeta potential, morphology, and transmission electron microscopy (TEM) to improve their cytotoxic effect against colorectal cancer metastasis. Quercetin-loaded, lecithin-chitosan nanoparticles (QU L/CH NPs) presented a well-defined spherical morphology with a mean size of 200 nm ± 42.4 nm and a zeta potential of +41.4 ± 1.60 mV. Using the 3,4,5-dimethylthiazol-2,5 biphenyl tetrazolium bromide (MTT) assay, we observed that QU L/CH NPs showed a significant cytotoxic effect against human colorectal cancer cells. QU L/CH NPs also induced apoptosis in CACO-2 cells by reducing the expression of genes correlated with signaling pathways in colorectal cancer such as HES-1 and HEY-1. These results suggest that quercetin-loaded, lecithin-chitosan nanoparticles have excellent potential for clinical application against human colorectal cancer. |
| Keywords |
| Quercetin-Colorectal Cancer-Lecithin/Chitosan Nanoparticles |
| Status: Abstract Accepted (Poster Presentation) |