| Interaction of silver nanoparticles with Bovine Serum Albumin |
| Paper ID : 1002-ISCH |
| Authors |
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Eman Fekry Abbas *1, Magdy Mohamed Khalil2, Said Moustafa3 1Medical Biophysics, Department of Physics, Faculty of Science, Helwan University, Cairo, Egypt. 2School of Applied Health Sciences, Badr University in Cairo (BUC), Badr City and Department of Physics, Faculty of Science, Helwan University, Cairo, Egypt. 3Solid state, Department of Physics, Faculty of Science, Helwan University, Cairo, Egypt. |
| Abstract |
| Aim: This study aims to investigate the interaction between silver nanoparticles ( AgNPs) and bovine serum albumin (BSA) as well as to examine the cytotoxic effect of AgNPs and its BSA conjugates on HepG2 cell as a cancer cell model. Methods: The prepared AgNPs were characterized by UV-Visible spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), then the interaction between AgNPs and BSA was studied by means of multiple spectral methods. The cytotoxic effect of Synthesized AgNPs and AgNPs-BSA conjugate against HepG2 cells was studied using MTT viability assay. Results: XRD pattern showed the face centered cubic structure of AgNPs. The TEM images showed the average particle size of citrate-coated AgNPs a value of 48 nm that agree with DLS results. UV-Vis and fluorescence spectroscopic studies showed that AgNPs form complex with BSA. Cytotoxicity results revealed that AgNPs were non-cytotoxic at low concentrations. As the concentration of AgNPs increased cytotoxicity tended to rise, our results also showed that adding BSA to AgNPs enhances the viability of HepG2 cells and decreases the toxicity of AgNPs on HepG2 cells by 18%. Conclusion: In this study, silver nanoparticles have been successfully synthesized by chemical reduction method. Fluorescence spectroscopy confirmed that AgNPs – BSA bio-conjugates induced conformational changes in bovine serum albumin. Cytotoxicity results revealed that AgNPs can inhibit the growth and viability of cancer cells (HepG2 cells). |
| Keywords |
| silver nanoparticles, bovine serum albumin, cytotoxicity, HepG2 cells. |
| Status: Abstract Accepted (Poster Presentation) |